The main study applied on the biochemical effects of probiotics on Hepatic Lipid Metabolism experimentally induced Nonalcoholic fatty liver disease (NAFLD) in rats. Thirty male albino rats were divided into three groups (10 rats each). The first group fed a normal diet and represents the control group. The second group (NAFLD) fed normal diet enriched with1% cholesterol and 2% coconut oil and act as positive control (+ ve control). The third group fed on normal diet enriched with1% cholesterol and 2% coconut oil and probiotics(BIO-BC™) at a dose of 1- 2g/liter at a rat dose (0.5-1ml/kg. body. weight). Samples collected after 2,4and6 weeks after induction from treatment. serum was collected for estimation of Total Cholesterol (TC) ,Triacyleglecerol (TAG), Low density lipoprotein cholesterol (LDL-C), Very low density lipoprotein cholesterol (VLDL-C), High density lipoprotein cholesterol (HDL-C), Total lipids (TL), phospholipids, Nonesterified fatty acids(NEFA),Apo-lipoprotein -B (Apo- B),Our Results revealed a. significant increase in serum TC, TAG, LDL-C, VLDL-C,T L, phospholipids, NEFA, Apo-B While serum HDL-C showed a significant decrease. The behavioral biochemical results indicated treatment with probiotics showed significant changes and improves these parameters.