The main objective of this study to investigate the alterations of chitosan on Hepatic function tests and Immunoglobulin in Experimental induced Nonalcoholic fatty liver disease [NAFLD] in rats. Thirty male albino rats were divided into three groups [10 rats each]. The first group fed a normal diet and represents the control group. The second group [NAFLD] fed normal diet enriched with1% cholesterol and 2% coconut oil and act as positive control [+ve control]. The third group fed on normal diet enriched with1% cholesterol and 2% coconut oil and chitosan to the basal diet at a dose 5%. Samples collected after 2,4and6 weeks after induction from treatment .serum was used for estimation of Liver Function Tests as Serum Aspartate aminotransferase [AST], Alanine aminotransferase [ALT], Gammaglutamyltransferase [GGT] and Immunoglobulins as Serum ImmunoglobulinA [IgA], Immunoglobulin G [IgG]. Our Results revealed a significantly increase in serum AST, ALT, GGT, Serum IgA, IgG were significantly increase was observed. The behavioral biochemical results indicated treatment with chitosan showed significant changes and improves these parameter