At the cell level, anthocyanins are synthesized in the cytoplasm and accumulated into the vacuole.
Hitherto, poor knowledge of these compounds limited factors for transportation through the tonoplast.
Recently, next-generation sequencing of the citrus genome allowed us to identify adaptive mechanism genes
due to expression with a high sequencing of genes encoding proteins Cs7g7300, Cs7g07300.1 and Cs7g07300.2.
One of the adaptive mechanism expression genes for H+ATPases controlling acidity resulting colour expression
was selected. In the present study, we tend to focus to study the identification and validation of DNA based
marker was carried out for proton pump in Citrus unshiu based on nine SSR primers which were specific to
evaluate the ability of the initial material for Mandarin fruits to distinguish whether acid-base proton pump
expressed normal fruits and red pulp fruits using a total of 18 DNA samples divided into two groups, each
group contain nine samples from normal Satsuma fruits and the second group contains nine samples from red
pulp. The result presented 12 polymorphic loci and 19 alleles, with 1.87 alleles per locus with genetic diversity
of 0.38. The genetic coefficient between these two groups (normal pulp and red pulp) was 0.36 on average,
ranging from 0.22 to 0.80. According to cluster formation, these samples could be divided into four sub-groups.
Furthermore, the locus association will be more beneficial to select the excellent plant materials through the
marker-assisted selection and for further genes research in the future. Generally, the variations in pulp colour
from yellow to dark yellow or red due to the level of acidity which controlling by the proton pump.