Staff Members - Benha University
Do we need to screen Egyptian voluntary blood donors for toxoplasmosis?
Asian Pacific Journal of Tropical Disease • 2016
Publication Information
Authors
Nagwa Mostafa El-Sayeda, , , , Maha Mohamed Abdel-Wahaba, Shereen Magdy Kishikb, Naglaa Fathy Alhusseinic
Keywords
oxoplasma gondii; Blood donors; ELISA; PCR
Journal
Asian Pacific Journal of Tropical Disease
Publisher
Elsevier (Singapore) Pte Ltd. All rights reserved.
Volume
6
Issue
4
Pages
260-264
publication.type
International
Paper Link
Open Link
Supplementary Materials
Not Available
Abstract
Abstract
Objective
To determine the value of voluntary blood donors screening in diagnosing asymptomatic toxoplasmosis in an attempt to reduce the risk of this infection in Egyptian immunocompromised recipients.
Methods
Serum samples from 300 healthy voluntary blood donors were analyzed for anti-Toxoplasma antibodies [immunoglobulin G (IgG) and immunoglobulin M (IgM)] using ELISA and detection of Toxoplasma gondii (T. gondii) parasitemia was done by real-time quantitative PCR (qrtPCR).
Results
Frequency of T. gondii infection in 300 healthy blood donors was 101 (33.67%), 10 (3.33%), 18 (6.00%) by ELISA IgG, IgM and qrtPCR, respectively. It was found that 8 of 18 (44.4%) donor samples positive by qrtPCR contained IgM anti-T. gondii, conversely 8 of 10 (80%) IgM-positive samples were positive for T. gondii DNA. There was a highly significant increase in detection of recent Toxoplasma infection using PCR over IgM ELISA by 55.6%. At the same time, T. gondii parasitemia was detected in 11 of 101 (10.90%) donor samples positive by IgG ELISA and in 7 of 199 (3.50%) negative donor samples for anti-T. gondii IgG antibodies. On the other hand, the negative results obtained by both qrtPCR and ELISA in 192 (64%) subjects ruled out the infection in those donors.
Conclusions
It might be appropriate to include the screening of blood and blood products for T. gondii in the pre-transfusion blood testing schedule in Egypt. Also, molecular screening should be carried out on the blood being transfused to immunocompromised patients.
Objective
To determine the value of voluntary blood donors screening in diagnosing asymptomatic toxoplasmosis in an attempt to reduce the risk of this infection in Egyptian immunocompromised recipients.
Methods
Serum samples from 300 healthy voluntary blood donors were analyzed for anti-Toxoplasma antibodies [immunoglobulin G (IgG) and immunoglobulin M (IgM)] using ELISA and detection of Toxoplasma gondii (T. gondii) parasitemia was done by real-time quantitative PCR (qrtPCR).
Results
Frequency of T. gondii infection in 300 healthy blood donors was 101 (33.67%), 10 (3.33%), 18 (6.00%) by ELISA IgG, IgM and qrtPCR, respectively. It was found that 8 of 18 (44.4%) donor samples positive by qrtPCR contained IgM anti-T. gondii, conversely 8 of 10 (80%) IgM-positive samples were positive for T. gondii DNA. There was a highly significant increase in detection of recent Toxoplasma infection using PCR over IgM ELISA by 55.6%. At the same time, T. gondii parasitemia was detected in 11 of 101 (10.90%) donor samples positive by IgG ELISA and in 7 of 199 (3.50%) negative donor samples for anti-T. gondii IgG antibodies. On the other hand, the negative results obtained by both qrtPCR and ELISA in 192 (64%) subjects ruled out the infection in those donors.
Conclusions
It might be appropriate to include the screening of blood and blood products for T. gondii in the pre-transfusion blood testing schedule in Egypt. Also, molecular screening should be carried out on the blood being transfused to immunocompromised patients.