Stem cell therapy is an important catigory of regenerative medicine has the potential to provide a valuable adjunct and alternative to liver transplantation with treatment of liver fibrosis and cirrhosis. In this approch, 70 male albino rats were randomly divided into 4 groups: Group 1: Control group (20 rats), Group 2: TAA-treated group (20 rats), Group 3:Mesenchymal stem cells treated group (15 rats) and Group 4: TAA- Mesenchymal stem cell –treated group (15 rats). Our results showed that TAA is significantly increase the activities of ALT, AST, and ALP and reduced the levels of total protein, albumin and globulins in the serum. Moreover, TAA successfully induced liver cirrhosis characterized by coarse nodular liver surface with severe fibrous connective tissue proliferation led to pseudolobulation of hepatic parenchyma. Meanwhile, after treatment with Mesenchymal stem cells (MSCs) the liver of the rats was grossly nearly normal and microscopically thin strands of fibrous connective tissue in between the hepatic lobules with mild degenerative and necrotic changes and significant improvement in the liver blood parameters. These findings concluded that MSCs have ameliorative effect on chemically induced liver cirrhosis through their regenerative capacity and antifibrotic effect.