Staff Members - Benha University
Bioinformatics and differential expression analysis of chalcone synthase genes (CHS1, 2, 3) under gamma rays elicitation in Silybum marianum L
4 th International Conference on Biotechnology Applications in Agriculture (ICBAA), Benha University, Moshtohor and Hurghada, 4-7 April 2018, Egypt • 2018
Publication Information
Authors
Sara M. Soliman, Hassan S. A. Sherif, Maria M. Bonfill, Hoda A. S. El-Garhy
Keywords
S. marianum, Gamma radiation, qRT-PCR, Sequencing, Chalcone synthase genes, Bioinformatics
Journal
4 th International Conference on Biotechnology Applications in Agriculture (ICBAA), Benha University, Moshtohor and Hurghada, 4-7 April 2018, Egypt
Publisher
Not Available
Volume
Not Available
Issue
Not Available
Pages
Not Available
publication.type
International
Paper Link
Not Available
Supplementary Materials
Not Available
Abstract
Silymarin is a constitutive secondary metabolite extracted from Silybum marianum seeds with wide therapeutic
and medical effects. Gamma irradiation could be a powerful tool for obtaining promising mutated genotypes of S.
marianum with high contents of flavonolignans. The transcript levels of the chalcone synthase genes (CHS1,
CHS2, and CHS3), encoded for silymarin, in response to two different gamma irradiation doses (200 and 600GY)
were determined using qRT-PCR. Also, Molecular isolation and characterization of chalcone synthase genes and
its encoded protein were investigated via bioinformatics tools. Results of qRT-PCR confirmed that exposure of S.
marianum seeds to gamma irradiation up-regulated the expression of the CHS1, CHS2 and CHS3 genes which
could be positively correlated with increasing of silymarin content in the fruits. In conclusion, the increasing of
flavonolignans content conflicts a great contribution in the field of medicine.
and medical effects. Gamma irradiation could be a powerful tool for obtaining promising mutated genotypes of S.
marianum with high contents of flavonolignans. The transcript levels of the chalcone synthase genes (CHS1,
CHS2, and CHS3), encoded for silymarin, in response to two different gamma irradiation doses (200 and 600GY)
were determined using qRT-PCR. Also, Molecular isolation and characterization of chalcone synthase genes and
its encoded protein were investigated via bioinformatics tools. Results of qRT-PCR confirmed that exposure of S.
marianum seeds to gamma irradiation up-regulated the expression of the CHS1, CHS2 and CHS3 genes which
could be positively correlated with increasing of silymarin content in the fruits. In conclusion, the increasing of
flavonolignans content conflicts a great contribution in the field of medicine.