The purpose of this study was to look into the antitumor activity of Asparagus racemosus root (AR) against hepatocellular carcinoma (HCC) in male rats that had been induced experimentally. Fifty white albino rats were categorized randomly to five groups. Group I act as a negative monitor. Group II was given DEN by gavage at dose of (20 mg/kg b.w/day/12 weeks). Group III rats were given AR (200 mg/kg b.w/18 weeks) via gavage. Group IV rats were given DEN at dose of (20 mg/kg b.w/day/12 weeks and then ARat dose of 200 mg/kg b.w/ 6 weeks. Group V rats were given AR at dose of (200 mg/kg b.w / 6 weeks before administration of DEN dose of (20 mg/kg b.w/day/12 weeks) till end of experiment. According to the findings, DEN treatment increased serum liver function enzymes ALT, AST, and ALP while decreasing the activity of hepatic antioxidant enzymes (SOD and CAT). DEN raised IL-6 levels while decreasing the apoptotic marker p53. Asparagus supplementation prior to or after DEN administration significantly reduced DEN toxicity and mutagenic effects, particularly when used as a protective. According to the findings, Asparagus racemosus roots should be used as an antioxidant and anti-tumor.