Nonalcoholic fatty liver disease (NAFLD) is sickness with different causes and it is the most
hepatic illness. The objective of this work is to investigate the bimolecular changes in a
model of high fat diet induced- NAFLD in rats. Also, the hypolipidemic, antioxidant and
anti-inflammatory effects of omega-3 were clarify via evaluation of some biochemical and
molecular parameters in the liver tissue of rats.Twenty one adult white albino rats were
divided into three groups. Group I (Normal control): received no drugs and fed balanced diet,
Group II (NAFLD- induced group): rats fed high fat diet for 8 weeks followed by normal
basal ration for additional 8 weeks. Group III (NAFLD + omega-3): rats administrated
omega-3 (300 mg/kg b.wt/day), orally for 8 weeks post-induction of NAFLD. The obtained
results showed a significant increase in serum Total cholesterol and Triacylglycerols (TAG)
concentrations, L-MDA and TAC in liver tissue with significant up-regulation in CPT-1α,
FAS, SREBP-1c, PPAR-α, Nrf2 and TAK1gene expression in NAFLD-induced rats.
Treatment with Omega-3 to NAFLD induced rats potentially improved molecular hepatic cell
function and oxidative alterations related to NAFLD near its normal ranges. Meanwhile,
histo-pathological findings supported that Omeg-3 markedly attenuates harmful effects of
NAFLD and protected liver cell. On conclusion, Omega-3 plays an important role as potent
anti-oxidant, anti-obesity, anti-inflammatory via inhibition of hepatic steatosis and oxidative
stress singling.