This work aimed to evaluate the probable protective effect of Moringa Oleifera on mycotoxins induced liver damage and oxidative stress in rats. Thirty male albino rats were classified randomly into three equal groups. Group1 (normal control): Rats fed with ordinary ration. Group2(Mycotoxin): Rats fed with ordinary diet only for first 7 days and from day 8 rats fed mixed ration contain 50 gram of grinded plant (total aflatoxins 4.2 ppb) + 50 gram of grinded fish (total aflatoxins 42.14 ppb) + 50 gram of ordinary diet (free of aflatoxins) for 4 weeks. Group3 (Mycotoxin+ Moringa): Moringa Oleifera (350 mg/kg b.w) was administered orally allover the experimental period (5 weeks). Also, rats were fed with ordinary ration only for first 7 days and from day 8 rats fed mixed ration that mentioned above in group 2. At the end of the experiment liver tissue specimens were isolated and analyzed for the determination of reduced Glutathione (GSH), superoxide dismutase (SOD), catalase (CAT), L-malondialdehyde (L-MDA) and tumor necrosis factor-alpha (TNF-α) gene expression. The obtained results showed a significant up-regulation of TNF-α gene expression level and marked increase in hepatic SOD, CAT activities and L-MDA of mycotoxin rats group. However, liver GSH concentration was markedly decreased as compared to control group. Moringa protected mycotoxins induced liver damage in rats caused a significant improvement of all previous parameters. Conclusively, Moringa Oleifera treatment exerts a protective effect against liver damage and oxidative stress in liver of mycotoxin intoxicated rats through free radical scavenging and anti-inflammatory activities as well as regenerating endogenous antioxidants defense system mechanisms.