The purpose of this study was to evaluate the biochemical parameters and cytokines levels in Egyptian patient suffering from Hepatitis C Virus. This study was conducted on 32 male. 25 of them were suffering from chronic HCV and the other 7 male are self-reported healthy volunteers. They were classified as the following: Group I (control healthy group), Group II (HCV-non treated group) Group III- Group VI (HCV-treated with 12,24,36,48 ampoule of interferon). The obtained results showed a significant increase in the activities of serum liver marker enzymes ALT, AST and ALP, pro-inflammatory cytokines, also a significant increase was observed in serum ferritin and alpha feto protein. On the other hand a significant decrease in both sera total protein and albumin level. Whereas administration of combination of pegylated IFN-α2b and ribavarin significantly improved the alert in the biochemical parameters. These results suggested that, combination therapy of both peg-interferon and ribavirin, emphasizing the influence of these compounds for patient with HCV, possibly preventing alteration of enzymes of liver activities. Because peg-interferon treatment can reduce liver injury and anti-inflammatory effect, as well as by decreasing plasma cytokine as cytokines, is present in patients with HCV liver disease. Furthermore, treatment with IFN-a diminishes the Th2 cytokine response. Thus, modulation of T-cell function and cytokine production may be one mechanism whereby IFN-α therapy results in reduced viral burden