The main objective of this study was to investigate the protective effect of vitamin E from oxidative stress induced by lipopolysaccharide (LPS, endotoxin) in rats. This study was carried out on 80 male rats. The rats were divided into four equal groups of 20 rats each. Group I (control): rats administered corn oil at dose 20 mg/kg/b.w/day for 5 weeks. Group II,rats administered vitamin E orally at dose 100 mg/kg/b.w/day for 5 weeks. Group III, rats injected intraperitoneally (i.p.)with a single dose of LPS at dose 20 mg / kg/b.w.Group IV, rats administered vitamin E at a dose 100 mg/kg/b.w/day for 5 weeks and then injected intraperitoneally (i.p.) with a single dose of LPS at a dose 20 mg / kg/b.w.Blood samples for serum separation and liver tissues were collected from all animal groups two times at 2 and 5 hours from the onset of injection with endotoxin. All sera were processed directly for determination of glucose,Uric Acid , total Cholesterol, Triacylglycerols, Phospholipids, FreeFatty Acid (FFA), Nitric oxide (NO), Aspartate aminotransferase (AST),Alanine aminotransferase ( ALT), ɤ-glutamyltransferase (GGT), Haptoglobin, α-1-acid glycoprotein (AGP), Vitamin C, Vitamin E, Reduced glutathione ( GSH) andL-Malondialdehyde (L-MDA) in addition to detrerminatuion of tissue Glutathione peroxidase (GPx), Superoxide dismutase (SOD), Catalase (CAT),Glutathione reductase (GR) ,Vitamin C,Vitamin E, GSH andL-MDA were also analyzed.The obtained results revealed that, endotoxemia could potentially increase serum Glucose, Uric Acid, Triacylglycerols,Phospholipid, Free Fatty acid,Haptoglobin, AGP,GSH,L- MDA concentrations in addition to ALT, AST and GGT activities. Moreover, the values of SOD, CAT,activities and GSH, L- MDA concentrations in liver tissueswere significantly decreased.Treatment with α- tocopherol (Vitamin E) decreased LPS-triggered pathogenic responses by mitigating liver damage and prevented the increase of lipid peroxidation (L-MDA),Glucose, Uric Acid, Triacylglycerols, Phospholipid, Free Fatty acid, ALT, GGT, Haptoglobin and AGP. Moreover, treatment with vitamin E in endotoxin injected rats responsible for the protection of membrane polyunsaturated fatty acids against lipid peroxidation by increasing the concentrations of serum and tissue GSH, SOD, CAT and Vitamin C. From the obtained results it could be concluded that, vitamin E protects against lipid peroxidation, oxidative stress and decrease the inflammatory response to endotoxin injection.

KEY WORDS: Endotoxin, Vitamin E, Oxidative stress, Inflammatory markers, Antioxidant status.