Staff Members - Benha University
Bacteriological and molecular detection of brucellosis with special reference to the effect of disinfectants on isolated strains.
BENHA VETERINARY MEDICAL JOURNAL • 2020
Publication Information
Authors
Lobna M. A. Salem 1, Nashwa O. Khalifa 1, Khoudair, R. M. 2 and Samar M. M. Moustafa 1
Keywords
brucellosis, isolation, PCR, disinfectants sensitivity
Journal
BENHA VETERINARY MEDICAL JOURNAL
Publisher
BENHA VETERINARY MEDICAL JOURNAL
Volume
VOL. 31,
Issue
Not Available
Pages
NO. 1:1-9,
publication.type
Local
Paper Link
Not Available
Supplementary Materials
Not Available
Abstract
A total of 127 specimens (13 aborted foeti, 46 milk samples, 37 lymph nodes, 14 livers, 14 spleen and 6 vaginal
discharges) were collected and examined for isolation and typing of Brucella microorganism. The results
detected 15 strains (5 aborted foeti, 4 milk, 5 lymph nodes and 1 spleen) were detected and typed as Br.
melitensis biovar 3. Application of PCR test for rapid identification of Brucella strains which isolated from
lymph nodes five of naturally infected animals (two cattle, one buffaloes, one sheep and one goat) revealed
that all samples were reacted positively with Br. melitensis specific DNA products with a molecular size of
731 pb. On sequencing, the Nucleotide sequence alignment of obtained sequences with other Brucella strain
indicated that the obtained isolate have high identity with Br. melitensis biovar 3. The bacteriocidal activity of
tested disinfectants against isolated Br. melitensis strain at variables concentration revealed that halogen
showed highest bactericidal activity followed by QACs and phenolic while alkaline wasthe lowest effect.
discharges) were collected and examined for isolation and typing of Brucella microorganism. The results
detected 15 strains (5 aborted foeti, 4 milk, 5 lymph nodes and 1 spleen) were detected and typed as Br.
melitensis biovar 3. Application of PCR test for rapid identification of Brucella strains which isolated from
lymph nodes five of naturally infected animals (two cattle, one buffaloes, one sheep and one goat) revealed
that all samples were reacted positively with Br. melitensis specific DNA products with a molecular size of
731 pb. On sequencing, the Nucleotide sequence alignment of obtained sequences with other Brucella strain
indicated that the obtained isolate have high identity with Br. melitensis biovar 3. The bacteriocidal activity of
tested disinfectants against isolated Br. melitensis strain at variables concentration revealed that halogen
showed highest bactericidal activity followed by QACs and phenolic while alkaline wasthe lowest effect.