The aim of this study was to study the application of counseling about umbilical cord stem cell collection and banking among pregnant women and its effect on their knowledge and attitude. Design: A quasi-experimental pre-post test design. Setting: The study was carried out at the antenatal clinics in Mansoura University Hospital, Egypt. Sample size: Ninety-eight pregnant women. Sample type: A purposive sampling. Tools: Counseling sheet using gather model, Likert scale, and woman satisfaction scale. Results: The study findings had revealed that there was a highly statistically significant improvement of knowledge about the umbilical cord stem cell collection and banking post-intervention compared to pre-intervention. Also, there was improvement among pregnant women concerning their attitude toward the umbilical cord stem cell collection and banking postintervention compared to pre-intervention. Further, there was a significant, positive correlation between women’s total score of knowledge and the total score of attitude pre and post-intervention. Conclusion: The present study results concluded that the application of counseling sessions resulted in a significant improvement among the pregnant women concerning their knowledge and attitude toward umbilical cord stem cell collection and banking. Therefore, it was recommended that a guideline and a brochure about umbilical cord blood stem cell collection and banking must be distributed among all pregnant women attending antenatal clinics.