Only a few conjugative plasmids from Mycobacteria have so far been identified and the assessment of their contribution to mycobacterial virulence is hampered by restricted knowledge of the functions of the plasmid genes. That also applies to the conjugative plasmid pRAW from Mycobacterium marinum. Bioinformatic tools nowadays facilitate prediction of gene functions. In this study we assigned COG (Clusters of Orthologous Groups of protein) groups to all 98 genes from pRAW including those that were annotated as “hypothetical” to contribute to functional characterization of genes from this conjugative plasmid.