Staff Members - Benha University
Environmental Mycobacterium avium subsp. hominissuis have a higher probability to act as a recipient in conjugation than clinical strains
Plasmid • 2018
Publication Information
Authors
Salma A. Shoulah, Anna M. Oschmann, Abdelfattah Selim, Torsten Semmler,
Carsten Schwarz, Elisabeth Kamal, Faysal Hamouda, Elsayed Galila, Wilbert Bitter,
Astrid Lewin
Keywords
Conjugation
Mycobacterium avium
Plasmid
Type I restriction modification
Antibiotic resistance
Journal
Plasmid
Publisher
Not Available
Volume
95
Issue
Not Available
Pages
28-35
publication.type
International
Paper Link
Not Available
Supplementary Materials
Not Available
Abstract
Mycobacterium avium subsp. hominissuis (MAH) is a widespread opportunistic pathogen that can be isolated from
environment (dust, soil and water) and patients with lung or lymphnode infection. In our previous research we
revealed the pronounced genetic diversity in MAH by identifying eight different types of a newly described
genomic island. In order to identify mechanisms of such horizontal gene transfer we now analyzed the ability of
47 MAH isolates to inherit the conjugative plasmid pRAW from M. marinum. A higher percentage of environmental isolates (22.7%) compared to clinical isolates (8%) had the capacity to function as recipient in conjugal
plasmid transfer. Genetic analysis showed additionally that environmental isolates contained more genes
homologous to genes present on conjugative mycobacterial plasmids than clinical isolates. Comparative analysis
of the genomes of the isolates pointed to a possible association between the ability to act as recipient in conjugation and the structure of a genomic region containing the radC gene and a type I restriction/modification
system. Finally we found that uptake of pRAW decreased the resistance against various antibiotics.
environment (dust, soil and water) and patients with lung or lymphnode infection. In our previous research we
revealed the pronounced genetic diversity in MAH by identifying eight different types of a newly described
genomic island. In order to identify mechanisms of such horizontal gene transfer we now analyzed the ability of
47 MAH isolates to inherit the conjugative plasmid pRAW from M. marinum. A higher percentage of environmental isolates (22.7%) compared to clinical isolates (8%) had the capacity to function as recipient in conjugal
plasmid transfer. Genetic analysis showed additionally that environmental isolates contained more genes
homologous to genes present on conjugative mycobacterial plasmids than clinical isolates. Comparative analysis
of the genomes of the isolates pointed to a possible association between the ability to act as recipient in conjugation and the structure of a genomic region containing the radC gene and a type I restriction/modification
system. Finally we found that uptake of pRAW decreased the resistance against various antibiotics.