QuEChERs method and HPLC were used to trace the recovery and the residue of Spinetoram (insecticide mixture of XDE-175-J and XDE-175-L, EPA Chemical Code: 110008 and 110009) which applied against the tomato leaf miner, Tuta absoluta (Meyrick) on or in tomato fruits. The experiment was carried out at El-Kharga Oasis New Valley, Egypt during early summer plantation of 2013. Spinetoram was sprayed on tomato at recommended dose and tomato fruit samples were collected at zero time (one hour after application), 1, 3, 5, 7, 10 and 15 days after application. Recoveries were ranged between 88.81- 95.41% with RSD of 3.4 -7.5% in tomato with fortification levels of 0.1, 0.5 and 1.0 mg / kg-¹ respectively. Limit of quantification (LOQ) of this method was found to be 0.1 mg kg-¹, while limit of detection was 0.005 mg kg-¹. Half-life (t½) and preharvest interval (PHI) were studied and they were 2.71 and 1 ٠ days