Two separate field experiments were conducted during 2010 and 2011 seasons, at the Research and Experimental Center of the Faculty of Agriculture at Moshtohor, Benha University, Kalubia Governorate, Egypt. The aim to study the effect of organic manure (sheep manure compost) alone or combined with mineral nitrogen fertilizer in the form ammonium nitrate 33.5%N and foliar application with Kinetin (a synthetic cytokinin b ppm) or Gibberellic acid (GA3 100 ppm) alone or mixture sprayed once at the squaring stage on growth attributes, yield and chemical composition of seeds as well as fiber quality of Egyptian cotton (Gossypium barbcedense L.) Giza 86 cotton cultivar. Each experiment included 12 treatments in every season. A Completely Randomized Block Design (CRBD) with four replicate was used in both seasons. The results could be summarized as follows: The highest ammonifying and nitrifying bacteria counts were in case of sheep manure compost-l-ammonium nitrate application at a rate of 30 kg N fed’ from each of them and sprayed with kinetin treatment. The application of sheep manure compost at rate of 30 kg N fed’ combined with 30 kg N fer’ mineral and sprayed by kinetin gave the highest values and significantly increased growth characters, yield and yield components, chemical composition of cotton seeds and fiber quality in both seasons. It can be concluded that the application of sheep manure compost at rate of 30 kg N fed’ combined with 30 kg N fed’ mineral and sprayed by kinetin may be the recommended treatment to improve the productivity of Egyptian cotton (Giza 86 variety), under the conditions of the present study.