This study was to investigate the changes of physiological and anatomical in tomato plants infected with tomato yellow leaf curl virus (TYLCV) isolate. it was found the growth characteristic, cell membrane stability index, photosynthetic pigment,carbohydrate contents and related to mineral ions contents of tomato plants. As were decreased, when compared with healthy plants. While electrical leakage increased than healthy plants. The mesophyll cells of infected tomato plants appeared relatively small or without intercellular spaces the palisade have lacking chlorenchyma with thin cell walls and chlorenchyma cells contained several cavities. Also the upper epidermis is composed of tubular paranchyma cells covered by thin layer of cuticle, when compared with healthy plants. Infected cells developed the presence of large number of abnormal vascular bundles, cell wall, cytoplasm membrane, chloroplast, mitochondria and nuclei. The chloroplast showed slightly elongated with irregular rows of grana destructed regions in chloroplastids which does not organize into grana and thylakoid system. Deformation elongated and curved mitochondria. Nucleus with several dark stained bodies destructed.