Staff Members - Benha University
GROWTH CHARACTERISTICS OF LOW PATHOGENIC STRAIN OF AVIAN INFLUENZA VIRUS (H5N2) IN DIFFERENT CELL CULTURES
• 2021
Publication Information
Authors
Zeinab, R. A. Abo Ezza
, El-Bagoury, G.F.a
, Khodier, M.H.b
and Sharawi, S.S.Aa
Keywords
Not Available
Journal
Not Available
Publisher
Not Available
Volume
Not Available
Issue
Not Available
Pages
Not Available
publication.type
Local
Paper Link
Not Available
Supplementary Materials
Not Available
Abstract
A low pathogenic strain of avian influenza virus (H5N2) was successfully propagated in both primary
chicken embryo fibroblast (CEF) cells and cell line of baby hamster kidney (BHK-21), African green
monkey kidney (Vero) and Madine Darby canine kidney (MDCK) cells through ten successive
passages; the virus induced cytopathic effect (CPE) from the 1st passage characterized by cell rounding,
elongation and cell disintegration. The most suitable cell cultures for virus propagation were MDCK
and BHK-21 cells that gave the highest titer 107.5 and 107 TCID50/ml respectively at the 10th passage
while the highest titer for Vero and CEF cells were 105.5 and 106.5 TCID50/ml respectively at the
10th passage. H5N2 virus was cell free virus with higher titer comparing to the cell associated virus in
both BHK-21 and MDCK cell line
chicken embryo fibroblast (CEF) cells and cell line of baby hamster kidney (BHK-21), African green
monkey kidney (Vero) and Madine Darby canine kidney (MDCK) cells through ten successive
passages; the virus induced cytopathic effect (CPE) from the 1st passage characterized by cell rounding,
elongation and cell disintegration. The most suitable cell cultures for virus propagation were MDCK
and BHK-21 cells that gave the highest titer 107.5 and 107 TCID50/ml respectively at the 10th passage
while the highest titer for Vero and CEF cells were 105.5 and 106.5 TCID50/ml respectively at the
10th passage. H5N2 virus was cell free virus with higher titer comparing to the cell associated virus in
both BHK-21 and MDCK cell line