Organizational citizenship behavior (OCB) and its connotation with organizational climate can be offered as a beneficial instrument for managers and authorities to determine the development of an attitude of associates and high interest in work and to improve the climate, values, and practices that characterize the members of a particular organization. The Aim of this Study: The aim of this study was to assess the relationship between OCB and organizational climate among nursing staff at Menoufia and Benha University Hospitals. Design: A descriptive-correlation research design was utilized. Setting: This study was conducted at different clinical units and departments at Menoufia and Benha University Hospital. Subjects: A convenience sample consisted of 365 nursing staff who are working in the previous setting. Tools: (a) Organizational climate description questionnaire and (b) Nurses’ organizational Citizenship Scale were used. Results: There was nearly half studied subject had the highest level of organizational citizenship behavior at Study setting while the highest level of organizational climate (good level) at Menoufia University Hospital than Benha University Hospital. And also there was a statistically significant difference regarding all dimensions of organizational climate and organizational citizenship behavior among studied Subject in the study setting. Conclusions: There was a positive correlation between organizational climate and OCB. Recommendations: It is recommended that change starts from the self; managers should be familiar with the organizational goals and be committed to achieve them to be good examples for staff and to create influence and motivation with their behavior