ABSTRACT The present study was carried out in the excremental to investigate the effect of collecting bee venom from Carniolan and Italian hybrid colony on brood rearing activity of such colonies. Data reveled that two hybrids collected a mean of 0.37 and 0.39 mg in freest year and of second year 0.36 and 0.39 mg for Carniolan and Italian hybrid respectively. The respectively sealed brood areas in the test colonies averaged 271.8 and 281.67 inch2/colony in 2012, and 283.81 and 267.38 inch2/colony in 2013 control colonies of both hybrids arised measurement ficantly sealed brood area in the two hybrids in both season. It is concluded that bee venom collecting did not affect significantly brood rearing activity.