In the present study, experiments of air infiltration are conducted on a prototype of cold store having different size entrances at three different cold store temperatures. The effects of cold store inside temperature (0, -5, and -7.5 oC), number of opened doors (entrance size), and opened door width and height on the magnitudes of infiltrated air and the associated infiltrated heat load are investigated. The experimental data are compared with the predicted data obtained from the computational fluid dynamics (CFD) model as well as the available published data. The comparison showed good agreement between them for the infiltrated air and infiltration heat load at 0 oC cold store temperature. While at -5 and -7.5 oC cold store temperatures, quite agreement is found between the measured and predicted data. The main target of the present study is to estimate the optimum air infiltration rate that helps in reducing the required thermal load and selecting the appropriate refrigeration machine. This helps promotion energy efficiency and minimizing the initial as well as the running costs of the cold store.