Background; Palliative care (PC) is specialized medical care for people living with a serious illness. PC is focused on providing relief from the symptoms and stress of the illness. The goal is to improve quality of life for both the patient and the family. Aim; This study aimed to evaluate the effect of palliative care guideline on nurses' knowledge, attitude and practice at intensive care unit. Design; Quasi experimental (pre/post test design) research design was used. Settings; general intensive care unit and emergency ICU at Benha University Hospital. Subjects; A convenience sample of all available nurses who are working in previous settings (No=100).Tools; I: Self-administered questionnaire for nurses to assess nurses'' knowledge regarding palliative care, II: attitude assessment scale and III: Nurses' observational Checklist to assess nurses ‘practices related to palliative care. Results; revealed that78.0% of the studied nurses age less than thirty years old , 44.0% had technical nursing institute and76.0% had an experience from 1