The study aimed to evaluate the effect of structured postpartum nursing intervention involving COVID-19 precautions on mothers' knowledge, practice, fear level and neonatal care. The study sample was purposive composed of 75 mothers during postpartum period. Study design was Quasi Experimental ( pre-post). Setting: the study conducted at the obstetrics and gynecology departments affiliated to University Hospital and Shebin El-Kom teaching hospital, Menoufia Governorate, Egypt. Five tools were used for data collection: a structured interviewing questionnaire, an observational checklist of mother practice of maternal and neonatal care, COVID-19 precautions knowledge questionnaire, Observational checklist of COVID-19 practice, and the COVID-19 fear scale. Study results revealed a statistically significant difference in the mothers' knowledge and practice regarding COVID-19 precautions and decrease in fear level on post and follow up intervention compared to pre intervention which indicated that the nursing intervention involving COVID-19 precautions had positive effect on mothers’ knowledge, practice and fear level. So, integrating postpartum nursing intervention involving COVID-19 precautions as a routine care for all postpartum mothers is recommended.