ABSTRACT This study was executed during two successive seasons 2014 and 2015 for evaluating the effect of inoculation with Bradyrhizobium sp, Bacillus mega-terium, Bacillus circulans, Glomus macrocarpum and Pseudomonas fluorescens on infection per-centage, enzymatic activity, growth characteristics, total phenol, peroxidase and polyphenol oxidase activity in presence or absence of Fusarium ox-ysporum in sterilized or non- sterilized soil under greenhouse condition. The obtained results ob-served that the tested strains showed suppression for F. oxysporum in vitro. Similar results were ob-tained under greenhouse experiment where the inoculation of soil with biofertilizers strains in pres-ence of P. fluorescens showed the lowest records of infection percentage as well as the higher val-ues of enzymatic activity, growth characteristics, total phenol, peroxidase and polyphenol oxidase activity compared to full dose of chemical fertilizers treatment. This study provides evidence for dimin-ishing the adverse evolved from the continues ap-plication of chemical fertilizers in high doses and replacing it (at least partially) through biofertilizers and biocontrol application.