Antibacterial activity of chitosan and some preservatives by disc diffusion method assay, inhibition percentage and minimal inhibitory concentration and Effect of MIC of chitosan on the survival of pathogenic bacterial strains compared to some preservatives were studied. Results showed that chitosan, sodium benzoate and sodium nitrite exhibited antibacterial activity against all the tested pathogens. Moreover, increasing the concentrations of chitosan and preservatives increased the antibacterial activity for E. coli, S. typhimurium, B. cereus and Staph. aureus. Also, inhibition percentage of all strains was increased with the increasing of fungal chitosan and preservatives concentrations. On the other hand, results showed that the counts of all pathogenic bacteria in the control treatment (without antibacterial agents) gradually increased with the increasing of incubation period to reach their maximum values after 42 h for E. coli and 48 h for S. typhimurium, Staph. aureus and B. cereus.