This research was carried out to study the efficiency of bio-control agents for controlling root-rot and wilting diseases of tomato. Obtained results showed that Trichoderma harzianum-l, Pseudomonas fluorescens-ll and Bacillus subtilis-1 were the best strains for controlling Rhizoctonia solani , Sclerotium rolfsii and Fuscirium oxysporum .sp lycopersici . Also, the seed dressing of tomato and soil drenching with bio-control agents gave the lowest records of disease severity of tomato, while, seed soaking only gave the highest records. Generally, it could be recormmended that the application of bio-control agents for tomato at sowing was more efficient for controlling of fungal soil borne diseases fungi. Application of such inocula minimizes the hazard effects of fungicides, protect the environment from pollution and maintenance of the human health.