Staff Members - Benha University
Association of interleukin (IL)-4 variable number of tandem repeats (VNTRs) and IL-4-590 promoter polymorphisms with susceptibility to pediatric autoimmune hepatitis type 1
• 2018
Publication Information
Authors
Amira Ibrahim Mansoura,⁎, Ola Galal Behairyb, Eman Rateb Abd Almonaemb,
Rasha Mahmoud Abd-Rabuhc, Inas Abd Elmonem Ahmedd
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publication.type
Local
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Abstract
Autoimmune hepatitis (AIH) is a chronic inflammatory liver disease mediated by an autoimmune
reaction to hepatocytes, the present study aimed to assess the possible associations between interleukin-4 (IL-4)
variable number of tandem repeats (VNTRs) and IL-4-590 promoter polymorphisms and susceptibility to autoimmune
hepatitis type 1 in children.
Subjects and methods: The study was performed on 101 children diagnosed with AIH and 104 apparently healthy,
age and sex-matched control children, diagnosis of AIH was based on the simplified score for the diagnosis of
AIH. Genotyping for the IL-4 VNTR and IL-4-590 were performed using PCR-RFLP.
Results: The distribution of genotype frequencies of IL-4 gene intron 3 VNTR polymorphism were not significantly
different between AIH patients and controls for 3R/2R and 2R/2R genotypes, while the 2R allele
distribution was significantly higher among AIH patients than the control group. The frequency of IL-4-590
single nucleotide polymorphism (SNP) CT and TT genotypes was statistically higher among AIH patients than
controls.
Conclusion: This study revealed the presence of an association between IL-4 -590 TT genotype and T alleles with
increased AIH risk in pediatric patients, also assess its severity as they were detected with Child Plugh scores B
and C.
reaction to hepatocytes, the present study aimed to assess the possible associations between interleukin-4 (IL-4)
variable number of tandem repeats (VNTRs) and IL-4-590 promoter polymorphisms and susceptibility to autoimmune
hepatitis type 1 in children.
Subjects and methods: The study was performed on 101 children diagnosed with AIH and 104 apparently healthy,
age and sex-matched control children, diagnosis of AIH was based on the simplified score for the diagnosis of
AIH. Genotyping for the IL-4 VNTR and IL-4-590 were performed using PCR-RFLP.
Results: The distribution of genotype frequencies of IL-4 gene intron 3 VNTR polymorphism were not significantly
different between AIH patients and controls for 3R/2R and 2R/2R genotypes, while the 2R allele
distribution was significantly higher among AIH patients than the control group. The frequency of IL-4-590
single nucleotide polymorphism (SNP) CT and TT genotypes was statistically higher among AIH patients than
controls.
Conclusion: This study revealed the presence of an association between IL-4 -590 TT genotype and T alleles with
increased AIH risk in pediatric patients, also assess its severity as they were detected with Child Plugh scores B
and C.