To our knowledge, the roles of kaempferol in semen extension or cryobiology haven’t been
discovered until now. Furthermore, the intracellular signals triggered by kaempferol in buffalo
spermatozoa during semen processing haven’t been reported. So, the current study intended to
clarify the role of kaempferol during the cryopreservation of buffalo spermatozoa. Six buffalo
bulls were adopted for semen collection. Two consecutive ejaculates were collected from each
buffalo bull weekly using an artificial vagina. The ejaculates were pooled to eliminate
variability. Semen extension was done using the Tris-based diluent supplemented with
Kaempferol at concentrations of 5, 10, 15, 25, 50, and 100 µg/ml vs. tris-based diluent only as
control. Samples were subjected to freezing then thawing to evaluate the vital sperm
characteristics. It was discovered that kaempferol at a concentration of 25 and 50 µg/ml of
significantly (p