The aim of this study was to investigate the effect of foliar application of bioregulators such as benzyladenine (BA), putrescine (Put) and salicylic acid (SA) at different concentrations on growth characteristics, biochemical compositions and yield of seeds and oil of sesame plant. The obtained results showed that the foliar application of BA (40 ppm), Put (100 ppm) and SA (50 ppm) induced high significant increase in all growth parameters (root and stem length, number and leaf area/plant, fresh and dry weights of roots, stems, leaves and whole plants, net assimilation rate (NAR) and fruiting zone length /plant). Biochemical composition represented in photosynthetic pigments, minerals, total sugars and carbohydrate, total free amino acids and crude protein contents in sesame leaves and seeds were also increased by this treatments. The foliar spray of BA (40 ppm). Put (100 ppm) and SA (50 ppm) significantly increased seeds yield and its components as well as seed oil yield /plant and pot, Also improved quality of fatty acids composition, a marked increase in unsaturated fatty acids and unsat : saturated ratios of sesame seeds oil.