Staff Members - Benha University
Toxicological Study on the Silver Nitrate Particles Synthesised by Nanotechnology Using Cell Culture and Apoptosis (Genetic and Cell study).
Indian Journal of Applied Research, • 2014
Publication Information
Authors
Ragia M. Hegazy,Eman Farouk, Taghreed G. Kharboush
Keywords
Not Available
Journal
Indian Journal of Applied Research,
Publisher
Not Available
Volume
4
Issue
(6):
Pages
1-8
publication.type
International
Paper Link
Not Available
Supplementary Materials
Not Available
Abstract
The present study focuses on the nanoparticle interaction with human peripheral blood cells from genetic and
ultra structure study. AgNPsgenotoxicity and cytotoxicity had been tested in human peripheral blood lymphocytes
culture through DNA fragmentation, chromosomal aberrations and electron microscope. Also, metal ion analysis
was done to check the uptake of nanoparticles in the cell pellet of human peripheral blood cells. AgNPs proved to be genotoxic
in human peripheral blood lymphocytes culture through the fragmentation of DNA, chromosomal aberrations and
metal ion analysis. Aberrant cell damage was recorded to be 0, 8 and 24% for 5, 15 and 25μg/mL of AgNPs respectively.
Significant increase and decrease in CAs and MI, respectively. Marked alterations in the ultrastructure blood cells in the form
of: apoptosis of leukocytes and destruction of erythrocytes membrane. Structural changes in chromosomes and chromatids
were also recorded. AgNPs was found to be cytotoxic and genotoxic.
ultra structure study. AgNPsgenotoxicity and cytotoxicity had been tested in human peripheral blood lymphocytes
culture through DNA fragmentation, chromosomal aberrations and electron microscope. Also, metal ion analysis
was done to check the uptake of nanoparticles in the cell pellet of human peripheral blood cells. AgNPs proved to be genotoxic
in human peripheral blood lymphocytes culture through the fragmentation of DNA, chromosomal aberrations and
metal ion analysis. Aberrant cell damage was recorded to be 0, 8 and 24% for 5, 15 and 25μg/mL of AgNPs respectively.
Significant increase and decrease in CAs and MI, respectively. Marked alterations in the ultrastructure blood cells in the form
of: apoptosis of leukocytes and destruction of erythrocytes membrane. Structural changes in chromosomes and chromatids
were also recorded. AgNPs was found to be cytotoxic and genotoxic.