Study On Free Living Amoebae (Acanthamoeba) In Association With Contact Lenses Mohamed H. Saleh, MD., Osman A. Salah El-Din and Ragaey R. Fahmy MD. Departments of Parasitology , ophthalmology and pathology, Benha faculty of medicine Benha University a b s t r a c t In Benha locality, free living amoebae (FLA) were isolated from 60% of 100 soft daily wear contact lenses, obtained from 50 contact lens wearers showing an improper lens care system and having proplems with their lenses. Acanthamoeba and Naegleria species represented 63.3% and 36.7% of isolated FLA strains respectively. The isolated FLA in the present study were non-pathogenic as proven by concanavalin A-induced agglutination test and experiemental animal pathogenivity test. Proper lens care is essential in patients using contact lenses. Research in Benha Med.J. 10 (3)(1993): 51–61.