Free living amoebae (FLA) were isolated from 33 (66%) out of 50 ear swabs (chronic ear infections) and from 21(42%) out of 50 nasal swabs (chronic nasal infections). Naegleria and Acanthamoeba species represented 39.4% and 60.6% respectively of 33 positive ear swabs and 33.3% and 66. 7% respectively of 21 positive nasal swabs. The percentage of Acanthamoeba species isolated from 54 positive ear and nasal swabs (63%) was higher than that of Naegleria species (37%). Naegleria infection was more acquired through aquatic source (swimming), white Acanthamoeba infection was more acquired through air and dust The incidence of isolation of FLA from both ear and nasal swabs was higher in females (78.2%) than in males (46.7%) and was higher in old age group (62.5%) than in middle and young age groups (50% and 58.3% respectively). The isolated PTA strains in the present study, either Naegleria or Acanthamoeba were non pathogenic as proven by animal pathogenicity test and played no rule in the pathogenesis and pathology of studied ear and nasal chronic infections.