Besides their protective role, polyamines also serve as signalling molecules. However, further studies are needed to elucidate the polyamine signalling pathways, especially to identify polyamine-regulated mechanisms and their connections with other regulatory molecules. Reduced height (Rht) genes in wheat are often used in breeding programs to increase harvest index. Some of these genes are encoding DELLA proteins playing role in gibberellic acid signalling. The aim of the present paper was to reveal how the mutations in Rht gene modify the polyamine-regulated processes in wheat. Wild type and two Rht mutant genotypes (Rht 1: semi-dwarf; Rht 3: dwarf mutants) were treated with polyamines. Polyamine treatments differently influenced the polyamine metabolism, the plant growth parameters and certain hormone levels (salicylic acid and abscisic acid) in these genotypes. The observed distinct …