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Comparative study between the protective role of Rosmarinic acid (RA) and the effect of mesenchimal stem cells on ameliorating the toxic effect of Duloxetine on the parotid gland of male albino rats. (histological and immunohistochemical study)
Zagazig Journal of Forensic Medicine and Toxicology • 2022
Publication Information
Authors
Amal M. Elsafy Elshazly1, Bodour Baioumy 1., Neama Mahmoud Taha 2, Asmaa Y.A. Hussein 3
and Yasmeen Mohammed Ismail El Sayed 4, and Osama foad 1.
Keywords
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Journal
Zagazig Journal of Forensic Medicine and Toxicology
Publisher
Not Available
Volume
Not Available
Issue
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Pages
1-18
publication.type
Local
Paper Link
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Supplementary Materials
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Abstract
Background: Duloxetine is an antidepressant drug utilized for treatment of anxiety disorders
and depressive disorders. This study aimed to compare the effect of Rosmarinic acid (RA) on the
parotid gland as natural antioxidant with the potential therapeutic effects of mesenchymal stem
cells on treating the toxic effect of Duloxetine on the parotid gland. Material & methods: 60
male albino rats were classified into 4 groups, 15 rats in each group. The first group (G1):control
group receive no treatment., group 2 (G2): received 10 mg/kg/d duloxetine for 12 week, group3
(G3): received Duloxetine the same as (G2) and received at the same time 120 mg/kg/d
Rosmarinic acid for 12 week, group4 (G4): received Duloxetine the same as (G2) then single
injection of MSCs just after stopping of Duloxetine , In all groups animals were sacrificed after
16 weeks following the beginning of the study. For histological evaluation, the parotid gland was
stained with hematoxylin and eosin, and Mallory trichrome stain was used for the demonstration
of collagen fibers. Also, Transmission electron microscope and immunohistochemistry using
caspas-3 were used to examine the parotid gland. Results: Examination of parotid sections in
G2 revealed inflammatory cells infiltration and massive distortion and vacuolation of serous
cells, however G3 and G4 showed marked improvement in the histological structure of the
parotid gland. Conclusion: MSCs and RA have good effect in decreasing the toxic effect of
Duloxetine administration on the parotid gland with insignificant difference between the two
methods
and depressive disorders. This study aimed to compare the effect of Rosmarinic acid (RA) on the
parotid gland as natural antioxidant with the potential therapeutic effects of mesenchymal stem
cells on treating the toxic effect of Duloxetine on the parotid gland. Material & methods: 60
male albino rats were classified into 4 groups, 15 rats in each group. The first group (G1):control
group receive no treatment., group 2 (G2): received 10 mg/kg/d duloxetine for 12 week, group3
(G3): received Duloxetine the same as (G2) and received at the same time 120 mg/kg/d
Rosmarinic acid for 12 week, group4 (G4): received Duloxetine the same as (G2) then single
injection of MSCs just after stopping of Duloxetine , In all groups animals were sacrificed after
16 weeks following the beginning of the study. For histological evaluation, the parotid gland was
stained with hematoxylin and eosin, and Mallory trichrome stain was used for the demonstration
of collagen fibers. Also, Transmission electron microscope and immunohistochemistry using
caspas-3 were used to examine the parotid gland. Results: Examination of parotid sections in
G2 revealed inflammatory cells infiltration and massive distortion and vacuolation of serous
cells, however G3 and G4 showed marked improvement in the histological structure of the
parotid gland. Conclusion: MSCs and RA have good effect in decreasing the toxic effect of
Duloxetine administration on the parotid gland with insignificant difference between the two
methods