Background: Male infertility is considered a major cause of couple infertility as it represents 25- 50
% of infertility cases. In addition, non-obstructive azoospermia (NOA) represents 10% of all infertile
men.There is no definite curable treatment for such cases.
Aim of Work: The purpose of this research was to assess the efficacy of BM-MSCs and AT-MSCs
in treating busulfan-induced azoospermia in wistar rats.
Materials and Methods: BM-MSCs were extracted from the femur bones of five adult Wistar rats
and AT-MSCs from preperitoneal adipose tissue. They were then evaluated for morphology, MSC
markers, osteogenic and adipogenic differentiation. To produce azoospermia in male Wistar rats,
two doses of busulfan (15 mg/kg and 30 mg/kg) were administered intraperitoneally during a 21-day
period. The seminiferous tubules of each testis were injected with 2.5106 MSCs 35 days after the
second busulfan injection. 12 weeks after cell therapy, the testes were examined histopathologically
and histomorphometrically.
Results: Like the control group, the seminiferous tubules treated with BM-MSCs demonstrated
normal spermatogenesis in compared to the busulfan-induced azoospermic testes. In contrast to
the busulfan-induced azoospermic testes, the seminiferous tubules treated with AT-MSCs exhibited