A total number of 150 matured Mamourah pullets aged 8 months were randomly grouped into six groups (25 pullets per each group). Pullets of the first three groups were artificially inseminated with semen of the same strain containing spermatozoa used as vectors for transferring level 20, 25 and 30µg of broiler hybrid-DNA (male) diluted with skim milk media in the presence of lipofectin reagent and considered as treated groups. Pullets of the other three groups were artificially inseminated with semen diluted with either media in the presence of Lipofectin reagent, semen diluted with only media or untreated semen and considered as control groups. It was aimed to study the effect of introducing broiler hybrid-DNA level into Mamourah embryonic cells by using spermatozoa as vectors on growth performance, feed efficiency and egg production over two successive generations.
Results obtained could be summarized as follows:
Highly significant (P