Depo provera is one of the most common used contraceptive. It is long acting synthetic progestin although it used as birth control it had little side effect on metabolism of body. Twenty eight albino female rats at breeding age were divided into two groups: (1) control group (8 animals) not injected by any drug. (2) Experimental group (20 animals) injected with Depo- provera (medroxyprogestrone acetate) (MPA) at a dose of 100mg/kg b.wt, respectively, through I/M monthly for 12 successive months. Blood samples collected from both groups every 3 months on heparin zed tubes for separation of plasma which used for determination of lipid profiles and erythrocytes preparation of haemolysates which subjected for estimation of antioxidant enzymes. Results showing a significant increase of total lipids and non significant increase of total cholesterol, triacyleglycerol, phospholipids, non esterified fatty acids, high density lipoprotein (HDL-c), low density lipoprotein (LDH-c), very low density lipoprotein (VLDL-c), lecithin acyle transferase (LCAT) and lipase enzyme. While IgG showing significant decrease at 12 month mean while IgM, IgA and erythrocytes antioxidant enzymes revealed a non significant decrease when compared to control group.