Gentamicin (GM) is a commonly prescribed antimicrobial drug used for treatment of infections but associated
hepatic and renal complications restrict its efficacy. Overproduction of free radicals and inflammation are
involved in GM-induced hepato-renal damage. Date palm is renowned to have antioxidant and anti-inflammatory
bioactive composites. In this context, the current research was purposed to assess the ameliorative influence of
date palm extract (DE) supplementation against GM-induced hepato-renal injury. Gas chromatography-mass
spectrometry (GC–MS) was used to detect the bioactive constitutes in DE. The protective action of high and
low doses of DE was assessed alongside the GM remediation (80 mg/kg) in rats. GM evoked significant alterations
in liver and kidney function biomarkers (aminotransferases, albumin, creatinine, and blood urea).
Furthermore, notable elevations in malondialdehyde (MDA) level and increment expression of inducible nitric
oxide synthase (iNOS) along with reduction in catalase (CAT) activity were observed in both organs after GM
treatment. Oxidative stress was the main modulatory mechanism in GM-induced hepato-renal toxicity. However,
DE could mitigate the GM-inflicted liver and kidney damage, in a dose-response pattern, due to its high content
of phenolics and flavonoids.