Staff Members - Benha University
The Regenerative Potentiality of Obestatin and Adipose Derived Stem Cells Microvesicles in Experimentally Induced Skeletal Muscle Injury: Histological and Immunohistochemical Study
• 2019
Publication Information
Authors
Nessrein Ibrahiem Salem1Omayma Kamel Helal1, Maysara Mahmoud Salem1, Dina Sabry Abdel Fatah2, , Esraa Hammad Mohamed Khirat1
Keywords
muscle injury, obestatin, microvesicles.
Journal
Not Available
Publisher
Not Available
Volume
Not Available
Issue
Not Available
Pages
Not Available
publication.type
International
Paper Link
Not Available
Supplementary Materials
Not Available
Abstract
Background
Acute muscle injury has been recognized as a global healthcare issue. obestatin derived from a polypeptide called preproghrelin and involved in muscle regeneration. Microvesicles (MVs) exist in almost all types of body fluid.
Aim of the work
To evaluate the regenerative and myogenic capabilities of both obestatin and adipose stem cell derived microvesicles in repairing rats skeletal muscle injury.
Material and Methods
Sixty-five adult male rats were divided into five groups. Group I: control group. Group II: twenty rats undergo thermal muscle injury, then subdivided into two subgroups; IIa & IIb. Group ΙIΙ (Obestatin): rats received injections of obestatin (30 μg/Kg) into tibialis anterior muscle each 24 hours for five days one week after muscle injury. Group ΙV (MVS): rats received a single dose of 15μg MVs into tibialis anterior muscle one week after muscle injury. Group V (Obestatin and MVS): rats received obestatin and MVs together (as in groups III & IV). Rats were sacrificed after four weeks after thermal injury (only group IIa sacrificed after one week) and specimens processed and examined using histological and immunohistochemical techniques.
Results
Group II (a & b) showed nearly the same histological picture of severe muscle disruption but there was a significant increase in collagen fibers deposition (P˂ 0.01) in groups IIb compared with group IIa. Group III and IV showed improvement in histological picture compared with group II. Group V showed a histological picture nearly similar to control group.
Conclusion:
Obestatin and Microvesicles (MVs) enhanced the repair of muscle injury. The effect of obestatin and microvesicles together is better than their use separately.
Acute muscle injury has been recognized as a global healthcare issue. obestatin derived from a polypeptide called preproghrelin and involved in muscle regeneration. Microvesicles (MVs) exist in almost all types of body fluid.
Aim of the work
To evaluate the regenerative and myogenic capabilities of both obestatin and adipose stem cell derived microvesicles in repairing rats skeletal muscle injury.
Material and Methods
Sixty-five adult male rats were divided into five groups. Group I: control group. Group II: twenty rats undergo thermal muscle injury, then subdivided into two subgroups; IIa & IIb. Group ΙIΙ (Obestatin): rats received injections of obestatin (30 μg/Kg) into tibialis anterior muscle each 24 hours for five days one week after muscle injury. Group ΙV (MVS): rats received a single dose of 15μg MVs into tibialis anterior muscle one week after muscle injury. Group V (Obestatin and MVS): rats received obestatin and MVs together (as in groups III & IV). Rats were sacrificed after four weeks after thermal injury (only group IIa sacrificed after one week) and specimens processed and examined using histological and immunohistochemical techniques.
Results
Group II (a & b) showed nearly the same histological picture of severe muscle disruption but there was a significant increase in collagen fibers deposition (P˂ 0.01) in groups IIb compared with group IIa. Group III and IV showed improvement in histological picture compared with group II. Group V showed a histological picture nearly similar to control group.
Conclusion:
Obestatin and Microvesicles (MVs) enhanced the repair of muscle injury. The effect of obestatin and microvesicles together is better than their use separately.