Liver samples were taken at 10 weeks for processing of paraffin sections, staining with H&E and Sirius red stain. The slides stained by Sirius red undergo quantitative analysis for collagen. Quantification was estimated by the percentage of stained area in comparison to the total area of fields examined. All data were expressed as mean area %. Difference between groups was compared by F-test with P < 0.05 selected as level of statistical significance. Also liver tissues were examined by immunohistochemistry for detection of MMP-13 and TIMP-1.
The mean area % for brown cytoplasmic staining (MMP-13 and TIMP-1) expression was quantified in 10 images for each in all groups