F1 wheat crosses having Yrl5 gene from the monogenic line (Triticum dicoccoides) were resistant stripe rust under greenhouse and field conditions at both seedling and adult stages. While, F2 populations segregated al ratio of 3 resistant: 1 susceptible which suggest that resistance to stripe rust is controlled by a single dominant gene. The successful confer of Yrl5 gene was emphasized by using the random amplified DNA polymorphism technique and the specific primer OPD-13. Among F2 populations of Yrl5×Sakha-69 (70 plants) and Yrl5×Giza-l63 (40 plants), the Yrl5 gene was detected in 52 and 29 plants, respectively with the ratio 3:1 Yrl5 gene was not detected in the tested wheat parents (Sakha-69 and Giza-163) nor in their susceptible F2 segregates.