Staff Members - Benha University
Zoonotic Hazards of Campylobacteriosis in some areas in Egypt
Life Science Journal • 2015
Publication Information
Authors
Barakat A. M. A. ; Mona M. Sobhy ; El Fadaly H. A. A.; Nagwa S. Rabie ; Nashwa O. Khalifa; Eman S. Ramadan; Kotb, M. H. R., Zeinab M. S. Amin Girh; Dalia M. Sedik;and Mona S. Zaki
Keywords
Campylobacter, milk product, fluorescence, prevalence, PCR
Journal
Life Science Journal
Publisher
Life Science Journal
Volume
12
Issue
7
Pages
9-14
publication.type
International
Paper Link
Open Link
Supplementary Materials
Not Available
Abstract
A total of 2130 samples collected from diarrhea chicken, raw milk, milk products and stool of patient
with diarrhea from Menia, Fayoum, Cairo and Qaluobya in Egypt. Samples were subjected to standard phenotypic
identification of C.jejuni, and subsequently immunofluorescent technique (IFT) identification and genetic
amplification by PCR using specific primers of hippuricase gene. The overall prevalence of Campylobacter jejuni in
intestine and liver of chicken were 40.4 % and 37.5 % respectively, 30% tape water, 4.44% raw milk, Karish cheese
and yoghurt 6.66% and 13.33% respectively and 70 (35%) children stool. The positive results of C.jejuni were
detected by IFT expressed by green fluorescence staining. PCR amplification of hipO gene of C. jejuni isolated from
the clinically diseased chicken and the environmental samples have shown identical fingerprints with human isolates
at 344bp, indicating the zoonotic hazards of Campylobacter jejuni in Egypt.
with diarrhea from Menia, Fayoum, Cairo and Qaluobya in Egypt. Samples were subjected to standard phenotypic
identification of C.jejuni, and subsequently immunofluorescent technique (IFT) identification and genetic
amplification by PCR using specific primers of hippuricase gene. The overall prevalence of Campylobacter jejuni in
intestine and liver of chicken were 40.4 % and 37.5 % respectively, 30% tape water, 4.44% raw milk, Karish cheese
and yoghurt 6.66% and 13.33% respectively and 70 (35%) children stool. The positive results of C.jejuni were
detected by IFT expressed by green fluorescence staining. PCR amplification of hipO gene of C. jejuni isolated from
the clinically diseased chicken and the environmental samples have shown identical fingerprints with human isolates
at 344bp, indicating the zoonotic hazards of Campylobacter jejuni in Egypt.