A total of 2130 samples collected from diarrhea chicken, raw milk, milk products and stool of patient with diarrhea from Menia, Fayoum, Cairo and Qaluobya in Egypt. Samples were subjected to standard phenotypic identification of C.jejuni, and subsequently immunofluorescent technique (IFT) identification and genetic amplification by PCR using specific primers of hippuricase gene. The overall prevalence of Campylobacter jejuni in intestine and liver of chicken were 40.4 % and 37.5 % respectively, 30% tape water, 4.44% raw milk, Karish cheese and yoghurt 6.66% and 13.33% respectively and 70 (35%) children stool. The positive results of C.jejuni were detected by IFT expressed by green fluorescence staining. PCR amplification of hipO gene of C. jejuni isolated from the clinically diseased chicken and the environmental samples have shown identical fingerprints with human isolates at 344bp, indicating the zoonotic hazards of Campylobacter jejuni in Egypt.