The present study was carried out to screen and analyze the characteristics of antibiotic resistance in Campylobacter strains isolated from human and chicken in the poultry farms of different localities in Egypt. A total of 340 samples were taken from human and various poultry farms and examined for bacteriologically for isolation of Campylobacter organisms. Fifty-six (16.47%) Campylobacter-positive using conventional method including 42 (12.35%) isolates for C. jejuni and 14 (4.12%) isolates for C. coli detected. Campylobacter isolates were evaluated for their antibiotic susceptibilities. Results of Antibiogram revealed that Campylobacter isolates were resistant to one or more of the antibiotics tested. Resistance was most frequently observed against streptomycin (96.4%) amoxicillin (94.6%), doxycycline (87.5%), Ampicillin (83.9%), nalidixic acid (85.7%), erythromycin and ciprofloxacin (82.1%). C. jejuni strains were often resistant to cephalothin (35.7%) than were C. coli strains (42.8%). C. coli were sensitive to erythromycin and Streptomycin (100%). C. jejuni was an increase sensitive to amoxicillin and streptomycin (95.2%). The trend of resistance to gentamicin (28.6%) and tetracycline (50%) was observed for C. jejuni. The present study provides an assessment of the occurrence of multidrug resistance of Campylobacter isolates from chicken samples collected from the poultry farms in different localities in Egypt. The antimicrobial resistance rates among these pathogens are clearly important in risk assessment and management. Further research is also needed to better understand the relationship between antimicrobial use in poultry and humans and the bacterial resistance in humans.