Staff Members - Benha University
Molecular Study of Campylobacter jejuni Isolated from Chicken, Dairy Cattle and Human to Determine Their Zoonotic Importance
• 2013
Publication Information
Authors
1 2 3 Nashwa O.Khalifa, Mervat E.I.Radwan and Mona, M.Sobhy 1 2 Dept. Zoonoses, Dept.Infectious Diseases, Fac.Vet.Med. Benha University.
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publication.type
International
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Abstract
Campylobacter jejuni is still the main causes of bacterial gastroenteritis worldwide. This work was done to investigate the fingerprinting of Campylobacter jejuni isolated from chicken, dairy cattle and human. Fecal samples were collected from 100 diarrheic chicken and dairy cattle (50 of each) as well as 50 stool samples from patients with diarrhea were subjected to standard isolation and identification of Campylobacter jejuni.
DNA of isolates was amplified using specific primer of hippuricase gene. The prevalence of Campylobacter jejuni was 18(36%) chicken, 16 (32%) dairy cattle and 11(22%) patients with diarrhea. PCR analysis produced identical bands at 344 bp in all isolates, indicating the role of chicken and dairy cattle in human Campylobacter infection
DNA of isolates was amplified using specific primer of hippuricase gene. The prevalence of Campylobacter jejuni was 18(36%) chicken, 16 (32%) dairy cattle and 11(22%) patients with diarrhea. PCR analysis produced identical bands at 344 bp in all isolates, indicating the role of chicken and dairy cattle in human Campylobacter infection