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Ameliorative effects of Green Tea Polyphenol Epigallocatechin-3-gallate on chlorpyrifos ovarian toxicity in adult albino rats
Mansoura Journal of Forensic Medicine and Clinical Toxicology • 2022
Publication Information
Authors
Hanaa E. Bayomy4 Amina A. Farag1* , Nashwa H. Abu-Raia2 , Arigue A. Dessouky
Keywords
Chlorpyrifos (CPF);
Green tea;
Epigallocatechin-3-gallate
(EGCG);
Oxidative stress
Journal
Mansoura Journal of Forensic Medicine and Clinical Toxicology
Publisher
Not Available
Volume
29
Issue
1110-5437
Pages
17-35
publication.type
Local
Paper Link
Open Link
Supplementary Materials
Not Available
Abstract
Chlorpyrifos (CPF) can induce ovarian damage and reproductive dysfunction
through oxidative stress mechanisms. These can be reversed by antioxidants such as
Epigallocatechin-3-gallate (EGCG) in green tea. This study aimed to investigate the
toxicity effects of CPF on rat ovaries and the effectiveness of EGCG to protect
against these effects. Fifty adult female albino rats were randomly assigned to five
equal groups; negative and positive control groups, EGCG-treated group, CPFtreated group and CPF+EGCG-treated group. After four weeks the rat body weight
and relative ovarian weight were estimated, and blood samples were collected to
assess reproductive hormones (RHs) levels. Evaluation of ovarian oxidative stress
indicators malondialdhyde (MDA), nitric oxide (NO), superoxide dismutase (SOD),
reduced glutathione (GSH) and catalase (CAT) was performed. Ovarian sections
were prepared and examined using the hematoxylin and eosin stain, proliferating cell
nuclear antigen, and morphometric study to assess histopathologic changes. CPFtreated rats had significant lower body weight and relative ovarian weight compared
to controls (P
through oxidative stress mechanisms. These can be reversed by antioxidants such as
Epigallocatechin-3-gallate (EGCG) in green tea. This study aimed to investigate the
toxicity effects of CPF on rat ovaries and the effectiveness of EGCG to protect
against these effects. Fifty adult female albino rats were randomly assigned to five
equal groups; negative and positive control groups, EGCG-treated group, CPFtreated group and CPF+EGCG-treated group. After four weeks the rat body weight
and relative ovarian weight were estimated, and blood samples were collected to
assess reproductive hormones (RHs) levels. Evaluation of ovarian oxidative stress
indicators malondialdhyde (MDA), nitric oxide (NO), superoxide dismutase (SOD),
reduced glutathione (GSH) and catalase (CAT) was performed. Ovarian sections
were prepared and examined using the hematoxylin and eosin stain, proliferating cell
nuclear antigen, and morphometric study to assess histopathologic changes. CPFtreated rats had significant lower body weight and relative ovarian weight compared
to controls (P