Staff Members - Benha University
Comparative study of the therapeutic effect of pomegranate alone or in combination with bone marrow mesenchymal stem cells on experimentally induced gastric ulcer in adult male rats: A histological and immunohistochemical study
The Egyptian journal of histology • 2018
Publication Information
Authors
Nahla El-Eraky El-Azab1, Aisha E. Mansy1, Abeer M. El-Mahalaway1, Dina Sabry
Keywords
Bone marrow mesenchymal stem cells (BMSCs), gastric ulcer, pomegranate, tumor necrosis factor-α, vascular
endothelial growth factor (VEGF).
Journal
The Egyptian journal of histology
Publisher
Not Available
Volume
Not Available
Issue
Not Available
Pages
Not Available
publication.type
International
Paper Link
Open Link
Supplementary Materials
Not Available
Abstract
Introduction: Gastric ulcers are the most prevalent gastrointestinal disorders. Pomegranate fruits have many beneficial
therapeutic effects. Bone marrow mesenchymal stem cells (BMSCs) are a novel approach with promising therapeutic potential
for curing human diseases.
Aim of the work: To evaluate the effect of pomegranate and BMSCs on experimentally induced gastric ulcer in adult male
rats.
Materials and Methods: Sixty adult male rats were divided into four groups. Group I kept as the control group. Group
II (Gastric ulcer), rats received 100% ethanol orally by gastric tube. Group (III) subdivided into 3 subgroups. Subgroup
IIIa included rats with gastric ulcer that treated with pomegranate only. Subgroup IIIb, rats with gastric ulcer treated
with BMSCs alone. Subgroup IIIc; gastric ulcer rats treated with a combination of pomegranate and BMSCs. Group IV
included the untreated gastric ulcer rats. Fundus of stomach specimens were prepared and examined using histological and
immunohistochemical techniques.
Results: Groups II and IV showed various changes as deep erosion, marked damage of most of the cells. The presence of the
inflammatory cells in gastric mucosa were supported by a significant increase of tumor necrosis factor α (TNF-α) expression
and collagen fibers depositions (P
therapeutic effects. Bone marrow mesenchymal stem cells (BMSCs) are a novel approach with promising therapeutic potential
for curing human diseases.
Aim of the work: To evaluate the effect of pomegranate and BMSCs on experimentally induced gastric ulcer in adult male
rats.
Materials and Methods: Sixty adult male rats were divided into four groups. Group I kept as the control group. Group
II (Gastric ulcer), rats received 100% ethanol orally by gastric tube. Group (III) subdivided into 3 subgroups. Subgroup
IIIa included rats with gastric ulcer that treated with pomegranate only. Subgroup IIIb, rats with gastric ulcer treated
with BMSCs alone. Subgroup IIIc; gastric ulcer rats treated with a combination of pomegranate and BMSCs. Group IV
included the untreated gastric ulcer rats. Fundus of stomach specimens were prepared and examined using histological and
immunohistochemical techniques.
Results: Groups II and IV showed various changes as deep erosion, marked damage of most of the cells. The presence of the
inflammatory cells in gastric mucosa were supported by a significant increase of tumor necrosis factor α (TNF-α) expression
and collagen fibers depositions (P