Background: Tramadol is an opioid pain medication used to treat premature ejaculation and as an
antidepressant also young population abuse tramadol. Long-term administration of tramadol has
negative impacts on many organs like ovary and testis. Objectives: The aim of study was to assess
effect of tramadol on testes and ovaries of adult albino rats and to elucidate possible protective effect of
Aloe Vera gel. Methodology: One hundred adult albino rats were used in present study. Animals were
divided into five groups: control group; solvent control group; Aloe Vera gel group, tramadol group
and tramadol and Aloe Vera gel group. Testes and ovaries were excised and specimens were processed
for light, and electron microscopic examination. Blood samples were obtained to be tested for:
testosterone, malondialdehyde (MDA), and reduced glutathione (GSH). Results: The present work
detected a highly significant reduction in serum testosterone, and GSH levels and a highly significant
increase in serum MDA levels in tramadol group as compared to control group. A highly significant
increase in serum testosterone, and GSH levels and a highly significant reduction in serum MDA levels
in tramadol and Aloe Vera gel group as compared to tramadol group and these results were nonsignificant
as compared to controls. Tramadol-treated male rats showed dilatation in seminiferous
tubules and vacuolation, testicular atrophy, and spermatogenic arrest, ovaries of tramadol-treated rats
showed atretic follicles, with hyperplasia of corpus luteum (CL) and vacuolation. All histopathological
changes, were ameliorated when Aloe Vera was co-administered with tramadol. Conclusion: Tramadol
has harmful effect on testes and ovaries of rats. Administration of Aloe Vera accelerates healing of
testicular tissue structure and has positive effect on testosterone level.