Staff Members - Benha University
Effect of Oxymatrine on Triclosan-Induced Ovarian Toxicity in Female Rats.
• 2021
Publication Information
Authors
Ali NE, Abd El kareem HM, Elnoury HA, Shalaby NM and Mahmoud AR
Keywords
Triclosan, Ovary, Oxidative stress, Oxymatrine and TNF‑α /MAPK/Caspase‑3 pathway.
Journal
Not Available
Publisher
Not Available
Volume
Not Available
Issue
Not Available
Pages
Not Available
publication.type
Local
Paper Link
Not Available
Supplementary Materials
Not Available
Abstract
Introduction: Triclosan [TCS] is a broad-spectrum antibacterial xenoestrogen
commonly used in cosmetics, soap and various products. Exposure to Triclosan
creates a disruption between reactive oxygen species (ROS) and antioxidant defenses.
Oxymatrine [OMT] has potent anti-cancer, anti-fibrosis, and anti-oxidant effects. Aim
of work: To study the effect of Oxymatrine on Triclosan-induced ovarian toxicity.
Material and Methods: The studied rats have been divided into five groups for
measurement of total antioxidant capacity, Caspase-3, the levels of Tumor Necrosis
Factor –alpha[TNF- α], Estrogen and Progesterone levels , and Liposaccharide Binding
Protein [LBP] gene expression in response to Triclosan-induced ovarian toxicity by realtime
Polymerase Chain Reaction [PCR]. Results: Triclolsan [TCS] caused statistically
significant reduction in total antioxidant capacity, with statistically significant elevation
in TNF-α and Caspase-3 activity compared to the control group. Oxymatrine induced
statistically significant elevation in total antioxidant capacity, statistically significant
decrease in the level of TNF-α, Caspase-3 activity with increased levels of Estrogen
and Progesterone compared to the Triclosan group. Histopathological and electron
microscope examination revealed vacuolar degeneration and atretic follicles in rats
treated with Triclosan, and significant improvement after Oxymatrine intake. In the
Triclolsan + Oxymatrine [TCS+OMT] group, gene expression levels of TNF‑α,
Caspase 3, Mitogen-activated protein kinase [MAPK], Nuclear Factor-kappa [NF-KB], and Liposaccharide Binding Protein [LBP] were statistically significant reduced compared
to the Triclolsan group.
commonly used in cosmetics, soap and various products. Exposure to Triclosan
creates a disruption between reactive oxygen species (ROS) and antioxidant defenses.
Oxymatrine [OMT] has potent anti-cancer, anti-fibrosis, and anti-oxidant effects. Aim
of work: To study the effect of Oxymatrine on Triclosan-induced ovarian toxicity.
Material and Methods: The studied rats have been divided into five groups for
measurement of total antioxidant capacity, Caspase-3, the levels of Tumor Necrosis
Factor –alpha[TNF- α], Estrogen and Progesterone levels , and Liposaccharide Binding
Protein [LBP] gene expression in response to Triclosan-induced ovarian toxicity by realtime
Polymerase Chain Reaction [PCR]. Results: Triclolsan [TCS] caused statistically
significant reduction in total antioxidant capacity, with statistically significant elevation
in TNF-α and Caspase-3 activity compared to the control group. Oxymatrine induced
statistically significant elevation in total antioxidant capacity, statistically significant
decrease in the level of TNF-α, Caspase-3 activity with increased levels of Estrogen
and Progesterone compared to the Triclosan group. Histopathological and electron
microscope examination revealed vacuolar degeneration and atretic follicles in rats
treated with Triclosan, and significant improvement after Oxymatrine intake. In the
Triclolsan + Oxymatrine [TCS+OMT] group, gene expression levels of TNF‑α,
Caspase 3, Mitogen-activated protein kinase [MAPK], Nuclear Factor-kappa [NF-KB], and Liposaccharide Binding Protein [LBP] were statistically significant reduced compared
to the Triclolsan group.