Staff Members - Benha University
Control of Tomato Fusarium wilt caused by Fusarium oxysporum f. sp. lycopersici by Grafting and Silver nanoparticles under greenhouse conditions
Benha Journal of Applied Sciences (BJAS) • 2022
Publication Information
Authors
Naeema.A.Gomaa, A.M.Mahdy, R.N.Fawzy, A.S.Mohamed and G.A.Ahmed
Keywords
Silver nanoparticles, grafting, Fusarium oxysporum, tomato ,enzymes , phenole.
Journal
Benha Journal of Applied Sciences (BJAS)
Publisher
Not Available
Volume
7
Issue
5
Pages
37-50
publication.type
Local
Paper Link
Not Available
Supplementary Materials
Not Available
Abstract
Grafting plays an important role in the management of pathogens infecting root and stem as this technology can be
quickly deployed without causing significant changes in farming operations. Grafting vegetable crops is common to control
fusarium wilt (caused by Fusarium oxysporum f.sp. lycopersici). Biological and chemical methods were used for the
synthesis of silver nanoparticles (AgNPs). AgNPs synthesized by two different methods i.e., Trichoderma harzianum, and
tri-sodium citrate. The synthesized silver nanoparticles were characterized with transmission electron microscopy (TEM),
Dynamic Light Scattering nano sizer (DLS) and Zeta potential. In vitro, Trichoderma harzianum AgNPs at concentration 800
µL/L inhibited completely the mycelium growth and sporulation of Fusarium oxysporum f.sp. lycopersici (FOL). Tri-sodium
citrate came in the second place for reducing the mycelium growth and sporulation of Fusarium oxysporum f.sp. lycopersici
(FOL). Results revealed the biological method was better than chemical method for controlling fusarium wilt caused by
(FOL). Grafted plants treated with silver nanoparticles exhibited an increase in activities of defense enzymes such as
peroxidase, polyphenol-oxidase, phenylalanine ammonia layse, chitinase and phenol contents over the control plants.
quickly deployed without causing significant changes in farming operations. Grafting vegetable crops is common to control
fusarium wilt (caused by Fusarium oxysporum f.sp. lycopersici). Biological and chemical methods were used for the
synthesis of silver nanoparticles (AgNPs). AgNPs synthesized by two different methods i.e., Trichoderma harzianum, and
tri-sodium citrate. The synthesized silver nanoparticles were characterized with transmission electron microscopy (TEM),
Dynamic Light Scattering nano sizer (DLS) and Zeta potential. In vitro, Trichoderma harzianum AgNPs at concentration 800
µL/L inhibited completely the mycelium growth and sporulation of Fusarium oxysporum f.sp. lycopersici (FOL). Tri-sodium
citrate came in the second place for reducing the mycelium growth and sporulation of Fusarium oxysporum f.sp. lycopersici
(FOL). Results revealed the biological method was better than chemical method for controlling fusarium wilt caused by
(FOL). Grafted plants treated with silver nanoparticles exhibited an increase in activities of defense enzymes such as
peroxidase, polyphenol-oxidase, phenylalanine ammonia layse, chitinase and phenol contents over the control plants.