Grafting plays an important role in the management of pathogens infecting root and stem as this technology can be quickly deployed without causing significant changes in farming operations. Grafting vegetable crops is common to control fusarium wilt (caused by Fusarium oxysporum f.sp. lycopersici). Biological and chemical methods were used for the synthesis of silver nanoparticles (AgNPs). AgNPs synthesized by two different methods i.e., Trichoderma harzianum, and tri-sodium citrate. The synthesized silver nanoparticles were characterized with transmission electron microscopy (TEM), Dynamic Light Scattering nano sizer (DLS) and Zeta potential. In vitro, Trichoderma harzianum AgNPs at concentration 800 µL/L inhibited completely the mycelium growth and sporulation of Fusarium oxysporum f.sp. lycopersici (FOL). Tri-sodium citrate came in the second place for reducing the mycelium growth and sporulation of Fusarium oxysporum f.sp. lycopersici (FOL). Results revealed the biological method was better than chemical method for controlling fusarium wilt caused by (FOL). Grafted plants treated with silver nanoparticles exhibited an increase in activities of defense enzymes such as peroxidase, polyphenol-oxidase, phenylalanine ammonia layse, chitinase and phenol contents over the control plants.